![Western blot analysis of lysates from wild type (WT) and MYL6B knockout (KO) 293T cells, using [KO Validated] MYL6B Rabbit polyclonal antibody (STJ11100075) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89145/357595/STJ11100075_1__06892.1713121734.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MYL6B knockout (KO) 293T cells, using [KO Validated] MYL6B Rabbit polyclonal antibody (STJ11100075) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western blot analysis of lysates from wild type (WT) and MYL6B knockout (KO) 293T cells, using [KO Validated] MYL6B Rabbit polyclonal antibody (STJ11100075) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89145/357595/STJ11100075_1__06892.1713121734.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MYL6B knockout (KO) 293T cells, using [KO Validated] MYL6B Rabbit polyclonal antibody (STJ11100075) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
Anti-MYL6B antibody (1-208) (STJ11100075)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal MYL6B (1-208) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:200-1:500ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MYL6B |
| Gene ID | 140465 |
| Uniprot ID | MYL6B_HUMAN |
| Immunogen Region | 1-208 |
| Immunogen Sequence | MPPKKDVPVKKPAGPSISKP AAKPAAAGAPPAKTKAEPAV PQAPQKTQEPPVDLSKVVIE FNKDQLEEFKEAFELFDRVG DGKILYSQCGDVMRALGQNP TNAEVLKVLGNPKSDELKSR RVDFETFLPMLQAVAKNRGQ GTYEDYLEGFRVFDKEGNGK VMGAELRHVLTTLGEKMTEE EVETVLAGHEDSNGCINYEA FLKHILSV |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-208 of human MYL6B (NP_002466.1). |
Additional Info
| Function | Regulatory light chain of myosin. Does not bind calcium. |
| Protein Name | Myosin Light Chain 6bMyosin Light Chain 1 Slow-Twitch Muscle A IsoformMlc1saSmooth Muscle And Nonmuscle Myosin Light Chain Alkali 6b |
| Database Links | Reactome: R-HSA-445355 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Myosin Light Chain 6b antibodyAnti-Myosin Light Chain 1 Slow-Twitch Muscle A Isoform antibodyAnti-Mlc1sa antibodyAnti-Smooth Muscle And Nonmuscle Myosin Light Chain Alkali 6b antibodyAnti-MYL6B antibodyAnti-MLC1SA antibody |
Information sourced from Uniprot.org