• Western blot analysis of extracts of normal 293T cells, 293T transfected with NLK Protein, using Myc-Tag rabbit monoclonal antibody antibody (STJ11103708) at 1:120000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of normal 293T cells, 293T transfected with NLK Protein and 293T transfected with PTEN Protein, using Myc-Tag antibody (STJ11103708) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of HeLa cells (Left: HeLa cells overexpressing recombinant protein with Myc-Tag；Right: Negative control without Myc-Tag) use rabbit anti Myc-Tag monoclonal antibody (STJ11103708) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300ug extract cell lysate from 293T cells transfected with NLK expression vector containing a Myc-Tag with 3 Mu g Myc-Tag rabbit monoclonal antibody antibody (STJ11103708). Western blot was performed from the immunoprecipitate using Myc-Tag rabbit monoclonal antibody antibody (STJ11103708) at 1:5000 dilution.
  • Immunoprecipitation analysis of 200ug extracts of 293T cells transfected with NLK expression vector containing a Myc-Tag with 3 Mu g Myc-Tag rabbit monoclonal antibody (STJ11103708 1:100). Western blot was performed from the immunoprecipitate using Myc-Tag rabbit monoclonal antibody (STJ11103708) at 1:1000 dilution.

Anti-Myc-Tag antibody [S8MR] (STJ11103708)

SKU:
STJ11103708

£96.50 - £392.50
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Host: Rabbit
Applications: WB/IF/ICC/IP/ELISA
Reactivity: Species independent
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:10000-1:120000
IF/ICC:1:50-1:200
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirem
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Specificity: A synthetic peptide corresponding to Myc tag.
Immunogen Sequence: EQKLISEEDL
Background Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.

Information sourced from Uniprot.org

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