| Short Description : | Rabbit polyclonal MVB12A (1-273) antibody for use in WB and ELISA in rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MVB12A |
| Gene ID: | 93343 |
| Uniprot ID: | MB12A_HUMAN |
| Immunogen Region: | 1-273 |
| Immunogen Sequence: | MDPVPGTDSAPLAGLAWSSA SAPPPRGFSAISCTVEGAPA SFGKSFAQKSGYFLCLSSLG SLENPQENVVADIQIVVDKS PLPLGFSPVCDPMDSKASVS KKKRMCVKLLPLGATDTAVF DVRLSGKTKTVPGYLRIGDM GGFAIWCKKAKAPRPVPKPR GLSRDMQGLSLDAASQPSKG GLLERTASRLGSRASTLRRN DSIYEASSLYGISAMDGVPF TLHPRFEGKSCSPLAFSAF |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human MVB12A (NP_612410.1). |
| Tissue Specificity | Ubiquitously expressed except in skeletal muscle. |
| Post Translational Modifications | Phosphorylated on Tyr-204 upon EGF stimulation. Phosphorylation is required for interaction with CD2AP and CIN85/SH3KBP1. |
| Function | Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in the ligand-mediated internalization and down-regulation of EGF receptor. |
| Protein Name | Multivesicular Body Subunit 12aCin85/Cd2ap Family-Binding ProteinEscrt-I Complex Subunit Mvb12aProtein Fam125a |
| Database Links | Reactome: R-HSA-162588Reactome: R-HSA-174490Reactome: R-HSA-917729Reactome: R-HSA-9610379Reactome: R-HSA-9615710 |
| Cellular Localisation | CytoplasmNucleusEndosomeCytoskeletonMicrotubule Organizing CenterCentrosomeLate Endosome MembranePeripheral Membrane ProteinColocalizes With F-ActinSome Fraction May Be Nuclear |
| Alternative Antibody Names | Anti-Multivesicular Body Subunit 12a antibodyAnti-Cin85/Cd2ap Family-Binding Protein antibodyAnti-Escrt-I Complex Subunit Mvb12a antibodyAnti-Protein Fam125a antibodyAnti-MVB12A antibodyAnti-CFBP antibodyAnti-FAM125A antibody |
Information sourced from Uniprot.org
