Anti-Mu-type opioid receptor/OPRM1/Mu OR nanobody [SAA2285] (STJN000676)
SPECIFICATIONS
ClonalityMonoclonal
HostHuman
ConjugationUnconjugated
IsotypeVHH-hFc
General Information
| Short Description | Human monoclonal nanobody anti-Mu-type opioid receptor/OPRM1/Mu OR is suitable for use in ELISA, Immunofluorescence and FConfocal Microscopy research applications. |
| Applications | ELISA/IF/FCM |
| Host | Human |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SAA2285 |
| Isotype | VHH-hFc |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Protein A/G purified from cell culture supernatant. |
| Dilution Range | ELISA: 1:2000-1:20000 |
| Formulation | 0.01M PBS, pH 7.4. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | Please contact with the lab for this information. |
Target Information
| Gene Symbol | Oprm1 |
| Gene ID | 18390 |
| Uniprot ID | OPRM_MOUSE |
Additional Info
| Function | Receptor for endogenous opioids such as beta-endorphin and endomorphin. Receptor for natural and synthetic opioids including morphine, heroin, DAMGO, fentanyl, etorphine, buprenorphin and methadone. Also activated by enkephalin peptides, such as Met-enkephalin or Met-enkephalin-Arg-Phe, with higher affinity for Met-enkephalin-Arg-Phe. Agonist binding to the receptor induces coupling to an inactive GDP-bound heterotrimeric G-protein complex and subsequent exchange of GDP for GTP in the G-protein alpha subunit leading to dissociation of the G-protein complex with the free GTP-bound G-protein alpha and the G-protein beta-gamma dimer activating downstream cellular effectors. The agonist- and cell type-specific activity is predominantly coupled to pertussis toxin-sensitive G(i) and G(o) G alpha proteins, GNAI1, GNAI2, GNAI3 and GNAO1 isoforms Alpha-1 and Alpha-2, and to a lesser extent to pertussis toxin-insensitive G alpha proteins GNAZ and GNA15. They mediate an array of downstream cellular responses, including inhibition of adenylate cyclase activity and both N-type and L-type calcium channels, activation of inward rectifying potassium channels, mitogen-activated protein kinase (MAPK), phospholipase C (PLC), phosphoinositide/protein kinase (PKC), phosphoinositide 3-kinase (PI3K) and regulation of NF-kappa-B. Also couples to adenylate cyclase stimulatory G alpha proteins. The selective temporal coupling to G-proteins and subsequent signaling can be regulated by RGSZ proteins, such as RGS9, RGS17 and RGS4. Phosphorylation by members of the GPRK subfamily of Ser/Thr protein kinases and association with beta-arrestins is involved in short-term receptor desensitization. Beta-arrestins associate with the GPRK-phosphorylated receptor and uncouple it from the G-protein thus terminating signal transduction. The phosphorylated receptor is internalized through endocytosis via clathrin-coated pits which involves beta-arrestins. The activation of the ERK pathway occurs either in a G-protein-dependent or a beta-arrestin-dependent manner and is regulated by agonist-specific receptor phosphorylation. Acts as a class A G-protein coupled receptor (GPCR) which dissociates from beta-arrestin at or near the plasma membrane and undergoes rapid recycling. Receptor down-regulation pathways are varying with the agonist and occur dependent or independent of G-protein coupling. Endogenous ligands induce rapid desensitization, endocytosis and recycling. Heterooligomerization with other GPCRs can modulate agonist binding, signaling and trafficking properties. Isoform 9: Isoform 9 is involved in morphine-induced scratching and seems to cross-activate GRPR in response to morphine. |
| Protein Name | Mu-Type Opioid ReceptorM-Or-1Mor-1 |
| Database Links | Reactome: R-MMU-111885Reactome: -MMU-202040Reactome: -MMU-375276Reactome: -MMU-418594 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCell ProjectionAxonPerikaryonDendriteEndosomeIs Rapidly Internalized After Agonist Binding |
| Alternative Nanobody Names | Anti-Mu-Type Opioid Receptor nanobodyAnti-M-Or-1 nanobodyAnti-Mor-1 nanobodyAnti-Oprm1 nanobodyAnti-Mor nanobodyAnti-Oprm nanobody |
Information sourced from Uniprot.org