• Western blot analysis of extracts of various cell lines, using MTX1 antibody (STJ110221) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. _Lysates/proteins: 25ug per lane. _Blocking buffer: 3% non-fat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.
  • Western blot analysis of Rat testis, using MTX1 Rabbit polyclonal antibody (STJ110221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunofluorescence analysis of U2OS cells using MTX1 antibody (STJ110221) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Confocal immunofluorescence analysis of U2OS cells using MTX1 Polyclonal Antibody (STJ110221) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-MTX1 antibody (186-435) (STJ110221)

SKU:
STJ110221

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MTX1 (186-435) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MTX1
Gene ID: 4580
Uniprot ID: MTX1_HUMAN
Immunogen Region: 186-435
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 186-435 of human MTX1 (NP_942584.2).
Immunogen Sequence: LKVHKISNPWQSPSGTLPAL RTSHGEVISVPHKIITHLRK EVHTFWIDTKNYVEVTRKWY AEAMPFPLNFFLPGRMQRQY MERLQLLTGEHRPEDEEELE KELYREARECLTLLSQRLGS QKFFFGDAPASLDAFVFSYL ALLLQAKLPSGKLQVHLRGL HNLCAYCTHILSLYFPWDGA EVPPQRQTPAGPETEEEPYR RRNQILSVLAGLAAMVGYAL LSGIVSIQRATPARAPGTR
Post Translational Modifications Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.
Function Involved in transport of proteins into the mitochondrion. Essential for embryonic development.
Protein Name Metaxin-1
Mitochondrial Outer Membrane Import Complex Protein 1
Database Links Reactome: R-HSA-1268020
Reactome: R-HSA-8949613
Reactome: R-HSA-9013404
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Mitochondrion Outer Membrane
Alternative Antibody Names Anti-Metaxin-1 antibody
Anti-Mitochondrial Outer Membrane Import Complex Protein 1 antibody
Anti-MTX1 antibody
Anti-MTX antibody
Anti-MTXN antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance