• Immunofluorescence analysis of U-2 OS cells using MTIF2 Rabbit polyclonal antibody (STJ118830) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using MTIF2 Rabbit polyclonal antibody (STJ118830) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using MTIF2 Rabbit polyclonal antibody (STJ118830) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of MTIF2 in paraffin-embedded human colon carcinoma using MTIF2 Rabbit polyclonal antibody (STJ118830) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MTIF2 in paraffin-embedded human lung cancer using MTIF2 Rabbit polyclonal antibody (STJ118830) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using MTIF2 Rabbit polyclonal antibody (STJ118830) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-MTIF2 antibody (428-727) (STJ118830)

SKU:
STJ118830

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MTIF2 (428-727) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MTIF2
Gene ID: 4528
Uniprot ID: IF2M_HUMAN
Immunogen Region: 428-727
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 428-727 of human MTIF2 (NP_002444.2).
Immunogen Sequence: GEEILEVESEPRAREVVDWR KYEQEQEKGQEDLKIIEEKR KEHKEAHQKAREKYGHLLWK KRSILRFLERKEQIPLKPKE KRERDSNVLSVIIKGDVDGS VEAILNIIDTYDASHECELE LVHFGVGDVSANDVNLAETF DGVIYGFNVNAGNVIQQSAA KKGVKIKLHKIIYRLVEDLQ EELSSRLPCAVEEHPVGEAS ILATFSVTEGKKKVPVAGCR VQKGQLEKQKKFKLTRNGH
Tissue Specificity Expressed in all tissues examined. Highest level in skeletal muscle.
Function One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.
Protein Name Translation Initiation Factor If-2 - Mitochondrial
If-2(Mt
If-2mt
If2(Mt
Database Links Reactome: R-HSA-5368286
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Translation Initiation Factor If-2 - Mitochondrial antibody
Anti-If-2(Mt antibody
Anti-If-2mt antibody
Anti-If2(Mt antibody
Anti-MTIF2 antibody

Information sourced from Uniprot.org

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