• Western blot analysis of extracts of NIH/3T3 cells, using MTAP antibody (STJ26168) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using MTAP antibody (STJ26168) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using MTAP rabbit polyclonal antibody (STJ26168) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using MTAP rabbit polyclonal antibody (STJ26168) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using MTAP rabbit polyclonal antibody (STJ26168) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HeLa cells using MTAP rabbit polyclonal antibody (STJ26168) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using MTAP rabbit polyclonal antibody (STJ26168) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using MTAP rabbit polyclonal antibody (STJ26168) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-MTAP antibody (1-283) (STJ26168)

SKU:
STJ26168

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MTAP (1-283) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MTAP
Gene ID: 4507
Uniprot ID: MTAP_HUMAN
Immunogen Region: 1-283
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human MTAP (NP_002442.2).
Immunogen Sequence: MASGTTTTAVKIGIIGGTGL DDPEILEGRTEKYVDTPFGK PSDALILGKIKNVDCVLLAR HGRQHTIMPSKVNYQANIWA LKEEGCTHVIVTTACGSLRE EIQPGDIVIIDQFIDRTTMR PQSFYDGSHSCARGVCHIPM AEPFCPKTREVLIETAKKLG LRCHSKGTMVTIEGPRFSSR AESFMFRTWGADVINMTTVP EVVLAKEAGICYASIAMATD YDCWKEHEEAVSVDRVLKT
Tissue Specificity Ubiquitously expressed.
Function Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.
Protein Name S-Methyl-5'-Thioadenosine Phosphorylase
5'-Methylthioadenosine Phosphorylase
Mta Phosphorylase
Mtap
Mtapase
Database Links Reactome: R-HSA-1237112
Reactome: R-HSA-8950505
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-S-Methyl-5'-Thioadenosine Phosphorylase antibody
Anti-5'-Methylthioadenosine Phosphorylase antibody
Anti-Mta Phosphorylase antibody
Anti-Mtap antibody
Anti-Mtapase antibody
Anti-MTAP antibody
Anti-MSAP antibody

Information sourced from Uniprot.org

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