• Western blot analysis of extracts of various cell lines, using MT-ND5 antibody (STJ110434) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunofluorescence analysis of paraffin-embedded Rat testis using MT-ND5 Rabbit polyclonal antibody (STJ110434) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat brain using MT-ND5 antibody (STJ110434) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MT-ND5 in paraffin-embedded human prostate cancer using MT-ND5 Rabbit polyclonal antibody (STJ110434) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat kidney using MT-ND5 antibody (STJ110434) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MT-ND5 in paraffin-embedded human colon carcinoma using MT-ND5 Rabbit polyclonal antibody (STJ110434) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using MT-ND5 antibody (STJ110434) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MT-ND5 in paraffin-embedded rat kidney using MT-ND5 Rabbit polyclonal antibody (STJ110434) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human prostate cancer using MT-ND5 antibody (STJ110434) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MT-ND5 in paraffin-embedded rat brain using MT-ND5 Rabbit polyclonal antibody (STJ110434) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using MT-ND5 Rabbit polyclonal antibody (STJ110434) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-MT-ND5 antibody (500-607) (STJ110434)

SKU:
STJ110434

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MT-ND5 (500-607) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MT-ND5
Gene ID: 4540
Uniprot ID: NU5M_HUMAN
Immunogen Region: 500-607
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 500-607 of mouse MT-ND5 (NP_904338.1).
Immunogen Sequence: IALELNNLTMKLSMNKANPY SSFSTLLGFFPSIIHRITPM KSLNLSLKTSLTLLDLIWLE KTIPKSTSTLHTNMTTLTTN QKGLIKLYFMSFLINIILII ILYSINLE
Function Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.
Protein Name Nadh-Ubiquinone Oxidoreductase Chain 5
Nadh Dehydrogenase Subunit 5
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Nadh-Ubiquinone Oxidoreductase Chain 5 antibody
Anti-Nadh Dehydrogenase Subunit 5 antibody
Anti-MT-ND5 antibody
Anti-MTND5 antibody
Anti-NADH5 antibody
Anti-ND5 antibody

Information sourced from Uniprot.org

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