• Western blot analysis of extracts of various cell lines, using MT-ND3 antibody (STJ119948) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using MT-ND3 antibody (STJ119948) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human appendix using MT-ND3 antibody (STJ119948) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using MT-ND3 antibody (STJ119948) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of L929 cells using MT-ND3 antibody (STJ119948) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HepG2 cells using MT-ND3 rabbit polyclonal antibody (STJ119948) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells using 3 Mu g MT-ND3 antibody (STJ119948). Western blot was performed from the immunoprecipitate using MT-ND3 antibody (STJ119948) at a dilution of 1:500.

Anti-MT-ND3 antibody (1-70) (STJ119948)

SKU:
STJ119948

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:100-1:500
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specifi
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MT-ND3
Gene ID: 4537
Uniprot ID: NU3M_HUMAN
Immunogen Region: 1-70
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1-70 of human MT-ND3 (YP_003024033.1).
Immunogen Sequence: MNFALILMINTLLALLLMII TFWLPQLNGYMEKSTPYECG FDPMSPARVPFSMKFFLVAI TFLLFDLEIA
Function Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity of complex I.
Protein Name Nadh-Ubiquinone Oxidoreductase Chain 3
Nadh Dehydrogenase Subunit 3
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Nadh-Ubiquinone Oxidoreductase Chain 3 antibody
Anti-Nadh Dehydrogenase Subunit 3 antibody
Anti-MT-ND3 antibody
Anti-MTND3 antibody
Anti-NADH3 antibody
Anti-ND3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance