• Anti-MT-ATP8 antibody (45-58) (STJ119897)
  • Immunofluorescence analysis of HeLa cells using MT-ATP8 Rabbit polyclonal antibody (STJ119897) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of MT-ATP8 in paraffin-embedded Human liver tissue using MT-ATP8 Rabbit polyclonal antibody (STJ119897) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using MT-ATP8 Rabbit polyclonal antibody (STJ119897) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MT-ATP8 antibody (45-58) (STJ119897)

SKU:
STJ119897

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MT-ATP8 (45-58) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MT-ATP8
Gene ID: 4509
Uniprot ID: ATP8_HUMAN
Immunogen Region: 45-58
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 45-58 of human MT-ATP8 (YP_003024030.1).
Immunogen Sequence: KNYNKPWEPKWTKI
Function Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.
Protein Name Atp Synthase Protein 8
A6l
F-Atpase Subunit 8
Database Links Reactome: R-HSA-163210
Reactome: R-HSA-8949613
Cellular Localisation Mitochondrion Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Atp Synthase Protein 8 antibody
Anti-A6l antibody
Anti-F-Atpase Subunit 8 antibody
Anti-MT-ATP8 antibody
Anti-ATP8 antibody
Anti-ATPASE8 antibody
Anti-MTATP8 antibody

Information sourced from Uniprot.org

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