• Immunofluorescence analysis of NIH/3T3 cells using MSP/MST1 Rabbit polyclonal antibody (STJ114829) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using MSP/MST1 Rabbit polyclonal antibody (STJ114829) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of MSP/MST1 in paraffin-embedded human placenta using MSP/MST1 Rabbit polyclonal antibody (STJ114829) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using MSP/MST1 Rabbit polyclonal antibody (STJ114829) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MST1 antibody (200-500) (STJ114829)

SKU:
STJ114829

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MST1 (200-500) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MST1
Gene ID: 4485
Uniprot ID: HGFL_HUMAN
Immunogen Region: 200-500
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 200-500 of human MSP/MST1 (NP_066278.3).
Immunogen Sequence: CREAACVWCNGEEYRGAVDR TESGRECQRWDLQHPHQHPF EPGKFLDQGLDDNYCRNPDG SERPWCYTTDPQIEREFCDL PRCGSEAQPRQEATTVSCFR GKGEGYRGTANTTTAGVPCQ RWDAQIPHQHRFTPEKYACK DLRENFCRNPDGSEAPWCFT LRPGMRAAFCYQIRRCTDDV RPQDCYHGAGEQYRGTVSKT RKGVQCQRWSAETPHKPQFT FTSEPHAQLEENFCRNPDG
Post Translational Modifications Cleaved after Arg-483, probably by HPN/Hepsin, to yield the active form consisting of two disulfide-linked chains.
Protein Name Hepatocyte Growth Factor-Like Protein
Macrophage Stimulatory Protein
Macrophage-Stimulating Protein
Msp Cleaved Into - Hepatocyte Growth Factor-Like Protein Alpha Chain - Hepatocyte Growth Factor-Like Protein Beta Chain
Database Links Reactome: R-HSA-8852405
Cellular Localisation Secreted
Alternative Antibody Names Anti-Hepatocyte Growth Factor-Like Protein antibody
Anti-Macrophage Stimulatory Protein antibody
Anti-Macrophage-Stimulating Protein antibody
Anti-Msp Cleaved Into - Hepatocyte Growth Factor-Like Protein Alpha Chain - Hepatocyte Growth Factor-Like Protein Beta Chain antibody
Anti-MST1 antibody
Anti-D3F15S2 antibody
Anti-DNF15S2 antibody
Anti-HGFL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance