Western blot analysis of extracts of OVCAR3 cells, using (STJ27944) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MSLN antibody (296-606) (STJ27944)

SKU:
STJ27944
£112.50 - £524.50
Free Shipping
Processing The item has been added
Enquire For Bulk Order

Covered by St John's Laboratory Guarantee

Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MSLN
Gene ID: 10232
Uniprot ID: MSLN_HUMAN
Immunogen Region: 296-606
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 296-606 of human Mesothelin (NP_037536.2).
Immunogen Sequence: EVEKTACPSGKKAREIDESL IFYKKWELEACVDAALLATQ MDRVNAIPFTYEQLDVLKHK LDELYPQGYPESVIQHLGYL FLKMSPEDIRKWNVTSLETL KALLEVNKGHEMSPQAPRRP LPQVATLIDRFVKGRGQLDK DTLDTLTAFYPGYLCSLSPE ELSSVPPSSIWAVRPQDLDT CDPRQLDVLYPKARLAFQNM NGSEYFVKIQSFLGGAPTED LKALSQQNVSMDLATFMKL
Tissue Specificity Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).
Post Translational Modifications Both MPF and the cleaved form of mesothelin are N-glycosylated. Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.
Function Membrane-anchored forms may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
Protein Name Mesothelin
Cak1 Antigen
Pre-Pro-Megakaryocyte-Potentiating Factor Cleaved Into - Megakaryocyte-Potentiating Factor
Mpf - Mesothelin - Cleaved Form
Database Links Reactome: R-HSA-163125
Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Golgi Apparatus
Megakaryocyte-Potentiating Factor: Secreted
Isoform 3: Secreted
Alternative Antibody Names Anti-Mesothelin antibody
Anti-Cak1 Antigen antibody
Anti-Pre-Pro-Megakaryocyte-Potentiating Factor Cleaved Into - Megakaryocyte-Potentiating Factor antibody
Anti-Mpf - Mesothelin - Cleaved Form antibody
Anti-MSLN antibody
Anti-MPF antibody

Information sourced from Uniprot.org

Related Products

Model Name Host Clonality Reactivity Application
STJ116034 Anti-MSLN antibody (296-606) (STJ116034) Rabbit Polyclonal Human/Mouse/Rat WB/IHC-P/IF/ICC/ELISA
STJA0010203 Anti-MSLN antibody (STJA0010203) Rabbit Polyclonal Human/Rat/Mouse WB/ELISA/IHC
STJA0010208 Anti-MSLN antibody (STJA0010208) Rabbit Polyclonal Human/Rat/Mouse WB/ELISA/IHC
STJ197037 Anti-MSLN antibody (400-500 aa) [ABT-MSLN] (STJ197037) Mouse Monoclonal Human WB/IHC
STJ115049 Anti-VPS39 antibody (606-875) (STJ115049) Rabbit Polyclonal Human/Mouse/Rat WB/IF/ICC/ELISA
STJ72688 Anti-ZNF652 antibody (594-606) (STJ72688) Goat Polyclonal Human/Cow/Pig Pep-ELISA/WB/IHC
STJ117999 Anti-MAP3K7 antibody (510-606) (STJ117999) Rabbit Polyclonal Human WB/ELISA
STJ11105588 Anti-BRAF antibody (507-606) (STJ11105588) Rabbit Polyclonal Human WB/ELISA
!