• Immunohistochemistry analysis of paraffin-embedded rat lung using Musashi-1 (Musashi-1 (MSI1) ) antibody (STJ111162) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Musashi-1 (Musashi-1 (MSI1) ) Polyclonal Antibody (STJ111162) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MSI1 antibody (200-362) (STJ111162)

SKU:
STJ111162

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MSI1 (200-362) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MSI1
Gene ID: 4440
Uniprot ID: MSI1H_HUMAN
Immunogen Region: 200-362
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 200-362 of human Musashi-1 (MSI1) (NP_002433.1).
Immunogen Sequence: SRVMPYGMDAFMLGIGMLGY PGFQATTYASRSYTGLAPGY TYQFPEFRVERTPLPSAPVL PELTAIPLTAYGPMAAAAAA AAVVRGTGSHPWTMAPPPGS TPSRTGGFLGTTSPGPMAEL YGAANQDSGVSSYISAASPA PSTGFGHSLGGPLIATAFTN GYH
Tissue Specificity Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.
Function RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-GAU(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
Protein Name Rna-Binding Protein Musashi Homolog 1
Musashi-1
Database Links Reactome: R-HSA-9010553
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Rna-Binding Protein Musashi Homolog 1 antibody
Anti-Musashi-1 antibody
Anti-MSI1 antibody

Information sourced from Uniprot.org

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