• Formalin-fixed, paraffin-embedded human colon carcinoma (Lynch) stained with anti-MSH-6 antibody using peroxidase-conjugate and DAB chromogen. Note the absence of nuclear staining of tumor cells whereas normal lymphocytes and stromal cells are positive.

Anti-MSH-6 antibody (374-540aa) [ZR342] (STJ180582)

SKU:
STJ180582

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Host: Rabbit
Applications: IHC-P
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-MSH-6 (374-540aa) is suitable for use in Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: ZR342
Conjugation: Unconjugated
Isotype: IgG
Formulation: Tris-HCI buffer containing stabilizing protein (BSA) and <0.1% ProClin
Purification: Affinity purified
Dilution Range: 1:100-200
Storage Instruction: Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C.
Gene Symbol: MSH6
Gene ID: 2956
Uniprot ID: MSH6_HUMAN
Immunogen Region: 374-540aa
Specificity: Positive Control: Colon carcinoma
Immunogen: Recombinant fragment (around aa 374-540) of human MSH6 protein
Post Translational Modifications The N-terminus is blocked. Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway.
Function Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.
Protein Name Dna Mismatch Repair Protein Msh6
Hmsh6
G/T Mismatch-Binding Protein
Gtbp
Gtmbp
Muts Protein Homolog 6
Muts-Alpha 160 Kda Subunit
P160
Database Links Reactome: R-HSA-5358565
Reactome: R-HSA-5632928
Reactome: R-HSA-5632968
Cellular Localisation Nucleus
Chromosome
Associates With H3k36me3 Via Its Pwwp Domain
Alternative Antibody Names Anti-Dna Mismatch Repair Protein Msh6 antibody
Anti-Hmsh6 antibody
Anti-G/T Mismatch-Binding Protein antibody
Anti-Gtbp antibody
Anti-Gtmbp antibody
Anti-Muts Protein Homolog 6 antibody
Anti-Muts-Alpha 160 Kda Subunit antibody
Anti-P160 antibody
Anti-MSH6 antibody
Anti-GTBP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance