• Immunofluorescence analysis of L929 cells using MRPS35 Rabbit polyclonal antibody (STJ118323) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of MRPS35 in paraffin-embedded Human colon carcinoma using MRPS35 Rabbit polyclonal antibody (STJ118323) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using MRPS35 Rabbit polyclonal antibody (STJ118323) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-MRPS35 antibody (1-323) (STJ118323)

SKU:
STJ118323

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MRPS35 (1-323) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MRPS35
Gene ID: 60488
Uniprot ID: RT35_HUMAN
Immunogen Region: 1-323
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human MRPS35 (NP_068593.2).
Immunogen Sequence: MAAAALPAWLSLQSRARTLR AFSTAVYSATPVPTPSLPER TPGNERPPRRKALPPRTEKM AVDQDWPSVYPVAAPFKPSA VPLPVRMGYPVKKGVPMAKE GNLELLKIPNFLHLTPVAIK KHCEALKDFCTEWPAALDSD EKCEKHFPIEIDSTDYVSSG PSVRNPRARVVVLRVKLSSL NLDDHAKKKLIKLVGERYCK TTDVLTIKTDRCPLRRQNYD YAVYLLTVLYHESWNTEEW
Protein Name Small Ribosomal Subunit Protein Ms35
28s Ribosomal Protein S28 - Mitochondrial
Mrp-S28
S28mt
28s Ribosomal Protein S35 - Mitochondrial
Mrp-S35
S35mt
Database Links Reactome: R-HSA-5368286
Reactome: R-HSA-5389840
Reactome: R-HSA-5419276
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Small Ribosomal Subunit Protein Ms35 antibody
Anti-28s Ribosomal Protein S28 - Mitochondrial antibody
Anti-Mrp-S28 antibody
Anti-S28mt antibody
Anti-28s Ribosomal Protein S35 - Mitochondrial antibody
Anti-Mrp-S35 antibody
Anti-S35mt antibody
Anti-MRPS35 antibody
Anti-MRPS28 antibody
Anti-HDCMD11P antibody
Anti-MDS023 antibody
Anti-PSEC0213 antibody

Information sourced from Uniprot.org

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