• Western blot analysis of the lysates from HeLa cells using MRPL32 antibody.
  • Immunofluorescence analysis of A549 cells, using MRPL32 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from MCF-7, HepG2, and Jurkat cells, using MRPL32 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-MRPL32 antibody (101-150) (STJ94222)

SKU:
STJ94222

£48.00 - £252.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Large ribosomal subunit protein bL32m (101-150) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MRPL32
Gene ID: 64983
Uniprot ID: RM32_HUMAN
Immunogen Region: 101-150
Specificity: MRP-L32 Polyclonal Antibody detects endogenous levels of MRP-L32 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from human MRPL32. AA range:101-150
Post Translational Modifications MRPL32 precursor is processed by the m-AAA protease (composed of AFG3L2 and SPG7), which cleaves the N-terminal transit peptide. Cleavage by the m-AAA protease takes place prior to assembly into the large subunit, an essential step for mitochondrial ribosome (mitoribosome) assembly. Proper processing by the m-AAA protease is dependent on the zinc-binding region within the tightly folded C-terminal domain of MRPL32: zinc-dependent folding halts degradation initiated from the N-terminus and triggers the release of mature MRPL32.
Function Component of the mitochondrial large ribosomal subunit (mt-LSU). The mitochondrial ribosome (mitoribosome) is a large ribonucleoprotein complex responsible for the synthesis of proteins inside mitochondria.
Protein Name Large Ribosomal Subunit Protein Bl32m
39s Ribosomal Protein L32 - Mitochondrial
L32mt
Mrp-L32
Database Links Reactome: R-HSA-5368286
Reactome: R-HSA-5389840
Reactome: R-HSA-5419276
Reactome: R-HSA-9837999
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Large Ribosomal Subunit Protein Bl32m antibody
Anti-39s Ribosomal Protein L32 - Mitochondrial antibody
Anti-L32mt antibody
Anti-Mrp-L32 antibody
Anti-MRPL32 antibody
Anti-HSPC283 antibody

Information sourced from Uniprot.org

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