• Immunoprecipitation analysis of 200 Mu g extracts of 293T cells using 1 Mu g MRPL28 antibody (STJ27702). Western blot was performed from the immunoprecipitate using MRPL28 antibody (STJ27702) at a dilution of 1:1000.
  • Immunofluorescence analysis of U2OS cells using MRPL28 Rabbit polyclonal antibody (STJ27702). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of MRPL28 in paraffin-embedded human gastric cancer using MRPL28 Rabbit polyclonal antibody (STJ27702) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MRPL28 in paraffin-embedded human lung cancer using MRPL28 Rabbit polyclonal antibody (STJ27702) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MRPL28 in paraffin-embedded rat ovary using MRPL28 Rabbit polyclonal antibody (STJ27702) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using MRPL28 Rabbit polyclonal antibody (STJ27702) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

Anti-MRPL28 antibody (1-256) (STJ27702)

SKU:
STJ27702

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MRPL28 (1-256) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MRPL28
Gene ID: 10573
Uniprot ID: RM28_HUMAN
Immunogen Region: 1-256
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-256 of human MRPL28 (NP_006419.2).
Immunogen Sequence: MPLHKYPVWLWKRLQLREGI CSRLPGHYLRSLEEERTPTP VHYRPHGAKFKINPKNGQRE RVEDVPIPIYFPPESQRGLW GGEGWILGQIYANNDKLSKR LKKVWKPQLFEREFYSEILD KKFTVTVTMRTLDLIDEAYG LDFYILKTPKEDLCSKFGMD LKRGMLLRLARQDPQLHPED PERRAAIYDKYKEFAIPEEE AEWVGLTLEEAIEKQRLLEE KDPVPLFKIYVAELIQQLQ
Tissue Specificity Found in a variety of normal tissues including spleen, testes, thymus, liver, kidney, brain, adrenal, lung and retinal tissue.
Protein Name Large Ribosomal Subunit Protein Bl28m
39s Ribosomal Protein L28 - Mitochondrial
L28mt
Mrp-L28
Melanoma Antigen P15
Melanoma-Associated Antigen Recognized By T-Lymphocytes
Database Links Reactome: R-HSA-5368286
Reactome: R-HSA-5389840
Reactome: R-HSA-5419276
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Large Ribosomal Subunit Protein Bl28m antibody
Anti-39s Ribosomal Protein L28 - Mitochondrial antibody
Anti-L28mt antibody
Anti-Mrp-L28 antibody
Anti-Melanoma Antigen P15 antibody
Anti-Melanoma-Associated Antigen Recognized By T-Lymphocytes antibody
Anti-MRPL28 antibody
Anti-MAAT1 antibody

Information sourced from Uniprot.org

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