Anti-MRPL12 antibody (31-80) (STJ94210)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from human MRPL12. AA range:31-80
General Information
| Short Description | Rabbit polyclonal anti-Large ribosomal subunit protein bL12m (31-80) for use in WB, ELISA and IHC in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA/IHC |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC-p 1:50-300ELISA 2000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MRPL12 |
| Gene ID | 6182 |
| Uniprot ID | RM12_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MRPL12. AA range:31-80 |
| Immunogen Region | 31-80 |
| Specificity | MRP-L12 Polyclonal Antibody detects endogenous levels of MRP-L12 protein. |
Additional Info
| Post Translational Modifications | Two mature forms are produced by differential two-step proteolytic cleavage. Cleaved by the mitochondrial processing protease to produce the long mature form and subsequently by the mitochondrial intermediate protease to produce the short mature form. In the presence of CUL3, undergoes 'Lys-63'-linked ubiquitination at Lys-150 which results in proteasomal degradation. |
| Function | As a component of the mitochondrial large ribosomal subunit, plays a role in mitochondrial translation. When present in mitochondria as a free protein not associated with the ribosome, associates with mitochondrial RNA polymerase POLRMT to activate transcription. Required for POLRMT stability. |
| Protein Name | Large Ribosomal Subunit Protein Bl12m39s Ribosomal Protein L12 - MitochondrialL12mtMrp-L125c5-2 Cleaved Into - Large Ribosomal Subunit Protein Bl12m - Long Mature Form - Large Ribosomal Subunit Protein Bl12m - Short Mature Form |
| Database Links | Reactome: R-HSA-5368286Reactome: R-HSA-5389840Reactome: R-HSA-5419276Reactome: R-HSA-9837999 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Large Ribosomal Subunit Protein Bl12m antibodyAnti-39s Ribosomal Protein L12 - Mitochondrial antibodyAnti-L12mt antibodyAnti-Mrp-L12 antibodyAnti-5c5-2 Cleaved Into - Large Ribosomal Subunit Protein Bl12m - Long Mature Form - Large Ribosomal Subunit Protein Bl12m - Short Mature Form antibodyAnti-MRPL12 antibodyAnti-MRPL7 antibodyAnti-RPML12 antibody |
Information sourced from Uniprot.org