Anti-MRAS antibody (109-208) [S4134RM] (STJ11104134)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Ras-Related Protein M-Ras (109-208) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S4134RM |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:100-1:500ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MRAS |
| Gene ID | 22808 |
| Uniprot ID | RASM_HUMAN |
| Immunogen Region | 109-208 |
| Immunogen Sequence | LILRVKDRESFPMILVANKV DLMHLRKITREQGKEMATKH NIPYIETSAKDPPLNVDKAF HDLVRVIRQQIPEKSQKKKK KTKWRGDRATGTHKLQCVIL |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 109-208 of human Mras (O14807). |
Additional Info
| Tissue Specificity | Expression highly restricted to the brain and heart. |
| Function | Signal transducer in the Ras-MAPK signaling pathway that regulates cell proliferation and survival. Core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex that regulates the MAPK pathway activation. The formation of the SMP complex only occurs when MRAS is GTP-bound. MRAS has low intrinsic GTPase activity and may require additional factors for activation. The SMP complex specifically dephosphorylates the inhibitory phosphorylation at 'Ser-259' of RAF1 kinase, 'Ser-365' of BRAF kinase and 'Ser-214' of ARAF kinase, stimulating their kinase activities. |
| Protein Name | Ras-Related Protein M-RasRas-Related Protein R-Ras3 |
| Database Links | Reactome: R-HSA-5673000Reactome: R-HSA-9726840Reactome: R-HSA-9726842 |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmic Side |
| Alternative Antibody Names | Anti-Ras-Related Protein M-Ras antibodyAnti-Ras-Related Protein R-Ras3 antibodyAnti-MRAS antibodyAnti-RRAS3 antibody |
Information sourced from Uniprot.org