• Western blot analysis of recombinant Monkeypox virus (MPXV) E8L protein, using Monkeypox virus (MPXV) E8L antibody (STJ11105015) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 10ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MPXV antibody (1-275 aa) [S5015RM] (STJ11105015)

SKU:
STJ11105015

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Monkeypox
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S5015RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:2000-1:6000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1-275 aa
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human Monkeypox virus (MPXV) E8L. (NP_536532.1).
Immunogen Sequence: MPQQLSPINIETKKAISDTR LKTLDIHYNESKPTTIQNTG KLVRINFKGGYISGGFLPNE YVLSTIHIYWGKEDDYGSNH LIDVYKYSGEINLVHWNKKK YSSYEEAKKHDDGIIIIAIF LQVSDHKNVYFQKIVNQLDS IRSANMSAPFDSVFYLDNLL PSTLDYFTYLGTTINHSADA AWIIFPTPINIHSDQLSKFR TLLSSSNHEGKPHYITENYR NPYKLNDDTQVYYSGEIIR
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance