Anti-MonoMethyl-Histone H3-Lys27 antibody [4K2-P7-G3] (STJA0033024)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG
ImmunogenPeptide
General Information
| Short Description | Mouse monoclonal anti-MonoMethyl-Histone H3-Lys27 for use in WB in All samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Mouse |
| Reactivity | All |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 4K2-P7-G3 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Chromatography |
| Dilution Range | WB 1:500-1:1000 |
| Formulation | Mouse IgG in phosphate buffered saline, glycerol and BSA |
| Storage Instruction | Store at 4Β°C short term. Aliquot and store at-20Β°C long term. Avoid freeze/thaw cycles. |
Target Information
| Immunogen | Peptide |
Additional Info
| Background | Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as βhistone codeβ. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone methylation occurs primarily at lysine and arginine residues on the amino terminal of core histones. Methylation of histones can either increase or decrease transcription of genes, depending on which amino acids (Lys or Arg) in the histones are methylated and how many methyl groups are attached (mono-, di-, tri-methylation on Lys, mono-di-symmetric/asymmetric methylation on Arg). Mostly, lysine methylation occurs primarily on histone H3 Lys4, 9, 27, 36, 79 and H4 Lys20, while Arginine methylation occurs primarily on histone H3 Arg2, 8, 17, 26 and H4 Arg3. Histone methylases (HMTs) and histone demethylases (HDMs) are major regulating factors. |
Information sourced from Uniprot.org