• Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K36 antibody (STJ23990) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H3-K36 Rabbit polyclonal antibody (STJ23990). Blue: DAPI for nuclear staining.
  • Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K36 antibody (STJ23990).
  • Western blot analysis of various lysates using MonoMethyl-Histone H3-K36 Rabbit polyclonal antibody (STJ23990) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Mono-Methyl-Histone H3-K36 antibody (32-42) (STJ23990)

SKU:
STJ23990

£129.50 - £639.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Mono-Methyl-Histone H3-K36 (32-42) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
IP 1:50-1:200
ChIP 1:20-1:100
ChIP-seq 1:20-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 32-42
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 32-42 of human histone H3 (NP_003520.1).
Immunogen Sequence: ATGGVKKPHRY
Background Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain. The protein is located in the cytoplasm. This gene contains a trinucleotide (GCT) repeat tract whose length is highly polymorphic; these triplet repeats result in a stretch of alanine residues in the encoded protein. Transcript variants utilizing alternative polyA signals and alternative 5 NA-terminal exons exist but all encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Information sourced from Uniprot.org

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