| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S5MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MME |
| Gene ID: | 4311 |
| Uniprot ID: | NEP_HUMAN |
| Immunogen Region: | 52-750 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 52-750 of human CD10/MME (NP_000893.2). |
| Immunogen Sequence: | YDDGICKSSDCIKSAARLIQ NMDATTEPCTDFFKYACGGW LKRNVIPETSSRYGNFDILR DELEVVLKDVLQEPKTEDIV AVQKAKALYRSCINESAIDS RGGEPLLKLLPDIYGWPVAT ENWEQKYGASWTAEKAIAQL NSKYGKKVLINLFVGTDDKN SVNHVIHIDQPRLGLPSRDY YECTGIYKEACTAYVDFMIS VARLIRQEERLPIDENQLAL EMNKVMELEKEIANATAKP |
| Post Translational Modifications | Myristoylation is a determinant of membrane targeting. Glycosylation at Asn-628 is necessary both for surface expression and neutral endopeptidase activity. |
| Function | Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Catalyzes cleavage of bradykinin, substance P and neurotensin peptides. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers. |
| Protein Name | Neprilysin Atriopeptidase Common Acute Lymphocytic Leukemia Antigen Calla Enkephalinase Neutral Endopeptidase 24.11 Nep Neutral Endopeptidase Skin Fibroblast Elastase Sfe Cd Antigen Cd10 |
| Database Links | Reactome: R-HSA-2022377 Reactome: R-HSA-5578768 Reactome: R-HSA-6798695 |
| Cellular Localisation | Cell Membrane Single-Pass Type Ii Membrane Protein |
| Alternative Antibody Names | Anti-Neprilysin antibody Anti-Atriopeptidase antibody Anti-Common Acute Lymphocytic Leukemia Antigen antibody Anti-Calla antibody Anti-Enkephalinase antibody Anti-Neutral Endopeptidase 24.11 antibody Anti-Nep antibody Anti-Neutral Endopeptidase antibody Anti-Skin Fibroblast Elastase antibody Anti-Sfe antibody Anti-Cd Antigen Cd10 antibody Anti-MME antibody Anti-EPN antibody |
Information sourced from Uniprot.org