Anti-MMACHC antibody (100-150 aa) (STJ194051)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human MMAC at the amino acid range 100-150
General Information
| Short Description | Rabbit polyclonal anti-Cyanocobalamin reductase/alkylcobalamin dealkylase (100-150 aa) for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MMACHC |
| Gene ID | 25974 |
| Uniprot ID | MMAC_HUMAN |
| Immunogen | Synthesized peptide derived from the human MMAC at the amino acid range 100-150 |
| Immunogen Region | 100-150 aa |
| Specificity | This antibody detects endogenous levels of MMAC at Human/Mouse |
Additional Info
| Function | Cobalamin (vitamin B12) cytosolic chaperone that catalyzes the reductive decyanation of cyanocob(III)alamin (cyanocobalamin, CNCbl) to yield cob(II)alamin and cyanide, using FAD or FMN as cofactors and NADPH as cosubstrate. Cyanocobalamin constitutes the inactive form of vitamin B12 introduced from the diet, and is converted into the active cofactors methylcobalamin (MeCbl) involved in methionine biosynthesis, and 5'-deoxyadenosylcobalamin (AdoCbl) involved in the TCA cycle. Forms a complex with the lysosomal transporter ABCD4 and its chaperone LMBRD1, to transport cobalamin across the lysosomal membrane into the cytosol. The processing of cobalamin in the cytosol occurs in a multiprotein complex composed of at least MMACHC, MMADHC, MTRR (methionine synthase reductase) and MTR (methionine synthase) which may contribute to shuttle safely and efficiently cobalamin towards MTR in order to produce methionine. Also acts as a glutathione transferase by catalyzing the dealkylation of the alkylcob(III)alamins MeCbl and AdoCbl, using the thiolate of glutathione for nucleophilic displacement to generate cob(I)alamin and the corresponding glutathione thioether. The conversion of incoming MeCbl or AdoCbl into a common intermediate cob(I)alamin is necessary to meet the cellular needs for both cofactors. Cysteine and homocysteine cannot substitute for glutathione in this reaction. |
| Protein Name | Cyanocobalamin Reductase / Alkylcobalamin DealkylaseAlkylcobalamin -Glutathione S-AlkyltransferaseCblcCyanocobalamin ReductaseCyanide-EliminatingMethylmalonic Aciduria And Homocystinuria Type C ProteinMmachc |
| Database Links | Reactome: R-HSA-3359473Reactome: R-HSA-3359474Reactome: R-HSA-9759218 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Cyanocobalamin Reductase / Alkylcobalamin Dealkylase antibodyAnti-Alkylcobalamin -Glutathione S-Alkyltransferase antibodyAnti-Cblc antibodyAnti-Cyanocobalamin Reductase antibodyAnti-Cyanide-Eliminating antibodyAnti-Methylmalonic Aciduria And Homocystinuria Type C Protein antibodyAnti-Mmachc antibodyAnti-MMACHC antibody |
Information sourced from Uniprot.org