• Immunohistochemistry analysis of MLLT4 in paraffin-embedded Rat kidney using MLLT4 Rabbit polyclonal antibody (STJ11102953) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using MLLT4 Rabbit polyclonal antibody (STJ11102953) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MLLT4 antibody (1510-1680) (STJ11102953)

SKU:
STJ11102953

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MLLT4 (1510-1680) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AFDN
Gene ID: 4301
Uniprot ID: AFAD_HUMAN
Immunogen Region: 1510-1680
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1510-1680 of human MLLT4 (NP_001353249.1).
Immunogen Sequence: SLSPDPWKRDAKEKLEKQQQ MHIVDMLSKEIQELQSKPDR SAEESDRLRKLMLEWQFQKR LQESKQKDEDDEEEEDDDVD TMLIMQRLEAERRARLQDEE RRRQQQLEEMRKREAEDRAR QEEERRRQEEERTKRDAEEK RRQEEGYYSRLEAERRRQHD EAARRLLEPEA
Function Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton. May play a key role in the organization of epithelial structures of the embryonic ectoderm. Essential for the organization of adherens junctions.
Protein Name Afadin
All1-Fused Gene From Chromosome 6 Protein
Protein Af-6
Afadin Adherens Junction Formation Factor
Database Links Reactome: R-HSA-418990 P55196-2
Cellular Localisation Cell Junction
Adherens Junction
Not Found At Cell-Matrix Ajs
Alternative Antibody Names Anti-Afadin antibody
Anti-All1-Fused Gene From Chromosome 6 Protein antibody
Anti-Protein Af-6 antibody
Anti-Afadin Adherens Junction Formation Factor antibody
Anti-AFDN antibody
Anti-AF6 antibody
Anti-MLLT4 antibody

Information sourced from Uniprot.org

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