• Western blot analysis of HCT116, using MLH3 Rabbit polyclonal antibody (STJ110106) at 1:3500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-MLH3 antibody (1190-1427) (STJ110106)

SKU:
STJ110106

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MLH3
Gene ID: 27030
Uniprot ID: MLH3_HUMAN
Immunogen Region: 1190-1427
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1190-1389 of human MLH3 (NP_055196.2).
Immunogen Sequence: QVLQQVDNKFIACLMSTKTE ENGEADSYEKQQAQGSGRKK LLSSTLIPPLEITVTEEQRR LLWCYHKNLEDLGLEFVFPD TSDSLVLVGKVPLCFVEREA NELRRGRSTVTKSIVEEFIR EQLELLQTTGGIQGTLPLTV QKVLASQACHGAIKFNDGLS LQESCRLIEALSSCQLPFQC AHGRPSMLPLADIDHLEQEK
Tissue Specificity Ubiquitous.
Function Probably involved in the repair of mismatches in DNA.
Protein Name Dna Mismatch Repair Protein Mlh3
Mutl Protein Homolog 3
Database Links Reactome: R-HSA-912446
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Dna Mismatch Repair Protein Mlh3 antibody
Anti-Mutl Protein Homolog 3 antibody
Anti-MLH3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance