• Human colon adenocarcinoma with Lynch syndrome stained with anti-MLH-1 antibody using peroxidase-conjugate and DAB chromogen. Note absence of nuclear staining of tumor cells whereas the background non tumor cells are positive.

Anti-MLH-1 antibody (Full length) [ZR347] (STJ180521)

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STJ180521

€214.38 - €856.88
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Host: Rabbit
Applications: IHC-P
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-MLH-1 (Full length) is suitable for use in Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: ZR347
Conjugation: Unconjugated
Isotype: IgG
Formulation: Tris-HCI buffer containing stabilizing protein (BSA) and <0.1% ProClin
Purification: Affinity purified
Dilution Range: 1:100-200
Storage Instruction: Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C.
Gene Symbol: MLH1
Gene ID: 4292
Uniprot ID: MLH1_HUMAN
Immunogen Region: Full length
Specificity: Positive control: Colon carcinoma
Immunogen: Full-length human MLH-1 protein
Tissue Specificity Colon, lymphocytes, breast, lung, spleen, testis, prostate, thyroid, gall bladder and heart.
Function Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.
Protein Name Dna Mismatch Repair Protein Mlh1
Mutl Protein Homolog 1
Database Links Reactome: R-HSA-5358565
Reactome: R-HSA-5358606
Reactome: R-HSA-5545483
Reactome: R-HSA-5632987
Reactome: R-HSA-6796648
Reactome: R-HSA-912446
Cellular Localisation Nucleus
Chromosome
Recruited To Chromatin In A Mcm9-Dependent Manner
Alternative Antibody Names Anti-Dna Mismatch Repair Protein Mlh1 antibody
Anti-Mutl Protein Homolog 1 antibody
Anti-MLH1 antibody
Anti-COCA2 antibody

Information sourced from Uniprot.org

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