• Fluorescence multiplex immunohistochemical analysis of Human tonsil tissue (formalin-fixed paraffin-embedded section). The immunostaining was performed by Pentuple-Fluorescence kit. CK-pan mouse monoclonal antibody (STJ197095 green), Ki-67 rabbit monoclonal antibody (STJA0006295) red, Iba 1 mouse monoclonal antibody (purple), CD8 a mouse monoclonal antibody (cyan), CD20 mouse monoclonal antibody (yellow), The section was incubated in 5 rounds of staining; sequentially for Anti-antibodies; each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (Excilone).

Anti-MKI67 antibody [ABT104R] (STJA0006295)

SKU:
STJA0006295

£139.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Proliferation marker protein Ki-67 is suitable for use in Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: ABT104R
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS pH7.4 containing 50% Glycerol and 0.03% Proclin 300.
Purification: The antibody was affinity-purified from cell supernatant by affinity-chromatography using specific immunogen.
Dilution Range: IHC-p 1:200-400
IF 1:200-400
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MKI67
Gene ID: 4288
Uniprot ID: KI67_HUMAN
Specificity: The antibody can specifically recognize human Ki-67 protein.
Immunogen: Synthesized peptide derived from human Ki-67
Post Translational Modifications Phosphorylated. Hyperphosphorylated in mitosis. Hyperphosphorylated form does not bind DNA.
Function Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. Does not contribute to the internal structure of mitotic chromosomes. May play a role in chromatin organization. It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
Protein Name Proliferation Marker Protein Ki-67
Antigen Identified By Monoclonal Antibody Ki-67
Antigen Ki-67
Antigen Ki67
Cellular Localisation Chromosome
Nucleus
Nucleolus
Associates With The Surface Of The Mitotic Chromosome
The Perichromosomal Layer
And Covers A Substantial Fraction Of The Mitotic Chromosome Surface
Associates With Satellite Dna In G1 Phase
Binds Tightly To Chromatin In Interphase
Chromatin-Binding Decreases In Mitosis When It Associates With The Surface Of The Condensed Chromosomes
Predominantly Localized In The G1 Phase In The Perinucleolar Region
In The Later Phases It Is Also Detected Throughout The Nuclear Interior
Being Predominantly Localized In The Nuclear Matrix
Alternative Antibody Names Anti-Proliferation Marker Protein Ki-67 antibody
Anti-Antigen Identified By Monoclonal Antibody Ki-67 antibody
Anti-Antigen Ki-67 antibody
Anti-Antigen Ki67 antibody
Anti-MKI67 antibody

Information sourced from Uniprot.org

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