• Biotinylated STJ70709 (0. 2µg/ml) staining of Daudi lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-MIF, Biotinylated antibody (C-Term) {Biotin} (STJ73265)

SKU:
STJ73265-100

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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-MIF, Biotinylated (C-Term) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Biotin
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.1-0.3µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: MIF
Gene ID: 4282
Uniprot ID: MIF_HUMAN
Immunogen Region: C-Term
Accession Number: NP_002406.1
Immunogen Sequence: NAANVGWNNSTFA.
Protein Name Macrophage Migration Inhibitory Factor
Mif
Glycosylation-Inhibiting Factor
Gif
L-Dopachrome Isomerase
L-Dopachrome Tautomerase
Phenylpyruvate Tautomerase
Database Links Reactome: R-HSA-202733
Reactome: R-HSA-6798695
Reactome: R-HSA-8950505
Cellular Localisation Secreted
Cytoplasm
Does Not Have A Cleavable Signal Sequence And Is Secreted Via A Specialized
Non-Classical Pathway
Secreted By Macrophages Upon Stimulation By Bacterial Lipopolysaccharide (Lps)
Or By M
Tuberculosis Antigens
Alternative Antibody Names Anti-Macrophage Migration Inhibitory Factor antibody
Anti-Mif antibody
Anti-Glycosylation-Inhibiting Factor antibody
Anti-Gif antibody
Anti-L-Dopachrome Isomerase antibody
Anti-L-Dopachrome Tautomerase antibody
Anti-Phenylpyruvate Tautomerase antibody
Anti-MIF antibody
Anti-GLIF antibody
Anti-MMIF antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance