| Host: | Goat |
| Applications: | Pep-ELISA/IHC |
| Reactivity: | Human/Mouse/Rat/Dog/Pig/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-MID2/TRIM1 (C-Term) is suitable for use in ELISA and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:4000.IHC: Paraffin embedded Human Kidney. Recommended concentration: 8µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | MID2 |
| Gene ID: | 11043 |
| Uniprot ID: | TRIM1_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_036348.2; NP_438112.2 |
| Specificity: | This antibody is expected to recognise both human isoforms of this protein. |
| Immunogen Sequence: | QESPYVSGMKTCH |
| Post Translational Modifications | Phosphorylated on serine and threonine residues. |
| Function | E3 ubiquitin ligase that plays a role in microtubule stabilization. Mediates the 'Lys-48'-linked polyubiquitination of LRRK2 to drive its localization to microtubules and its proteasomal degradation in neurons. This ubiquitination inhibits LRRK2 kinase activation by RAB29. |
| Protein Name | Probable E3 Ubiquitin-Protein Ligase Mid2Midin-2Midline Defect 2Midline-2Ring Finger Protein 60Ring-Type E3 Ubiquitin Transferase Mid2Tripartite Motif-Containing Protein 1 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule-Associated |
| Alternative Antibody Names | Anti-Probable E3 Ubiquitin-Protein Ligase Mid2 antibodyAnti-Midin-2 antibodyAnti-Midline Defect 2 antibodyAnti-Midline-2 antibodyAnti-Ring Finger Protein 60 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Mid2 antibodyAnti-Tripartite Motif-Containing Protein 1 antibodyAnti-MID2 antibodyAnti-FXY2 antibodyAnti-RNF60 antibodyAnti-TRIM1 antibody |
Information sourced from Uniprot.org