| Host: | Goat |
| Applications: | Pep-ELISA/WB/IHC |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-MIA/CD-RAP (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 14kDa band observed in Human Glioma lysates (calculated MW of 14.5kDa according to NP_006524.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.IHC: Par |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | MIA |
| Gene ID: | 8190 |
| Uniprot ID: | MIA_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_006524.1 |
| Specificity: | The immunizing peptide represents the N terminus of the mature protein. Reported variants represent identical protein: NP_006524.1, NP_001189482.1 |
| Immunogen Sequence: | PKLADRKLCADQE |
| Post Translational Modifications | May possess two intramolecular disulfide bonds. |
| Function | Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas. |
| Protein Name | Melanoma-Derived Growth Regulatory ProteinMelanoma Inhibitory Activity Protein |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Melanoma-Derived Growth Regulatory Protein antibodyAnti-Melanoma Inhibitory Activity Protein antibodyAnti-MIA antibody |
Information sourced from Uniprot.org
