Confocal imaging ofย paraffin-embedded Human liver usingย MGST1 Rabbit monoclonal antibody (STJ11102719, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-MGST1 antibody (1-100) [S9MR] (STJ11102719)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11102719
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General Information

Short DescriptionRabbit monoclonal MGST1 (1-100) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS9MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:1000-1:6000
IHC-P:1:200-1:2000
IF/ICC:1:200-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolMGST1
Gene ID4257
Uniprot IDMGST1_HUMAN
Immunogen Region1-100
Immunogen SequenceMVDLTQVMDDEVFMAFASYA TIILSKMMLMSTATAFYRLT RKVFANPEDCVAFGKGENAK KYLRTDDRVERVRRAHLNDL ENIIPFLGIGLLYSLSGPDP
SpecificityA synthetic peptide corresponding to a sequence within amino acids 1-100 of human MGST1 (P10620).

Additional Info

Tissue Specificity Highly expressed in liver.
Function Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
Protein Name Microsomal Glutathione S-Transferase 1
Microsomal Gst-1
Microsomal Gst-I
Database Links Reactome: R-HSA-156590
Reactome: R-HSA-5423646
Reactome: R-HSA-6798695
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Mitochondrion Outer Membrane
Alternative Antibody Names Anti-Microsomal Glutathione S-Transferase 1 antibody
Anti-Microsomal Gst-1 antibody
Anti-Microsomal Gst-I antibody
Anti-MGST1 antibody
Anti-GST12 antibody
Anti-MGST antibody

Information sourced from Uniprot.org

Citations

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