Western blot analysis of various lysates using Mfsd2a Rabbit polyclonal antibody (STJ11100244) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.

Anti-Mfsd2a antibody (170-245) (STJ11100244)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11100244
๐Ÿšš Free UK Delivery on orders over ยฃ150
Processing The item has been added
Enquire For Bulk Order
✓ 1-Year Performance Guarantee | SDS & Datasheet Included | Expert Tech Support | Covered by St John's Laboratory Guarantee

General Information

Short DescriptionRabbit polyclonal Mfsd2a (170-245) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolMfsd2a
Gene ID76574
Uniprot IDNLS1_MOUSE
Immunogen Region170-245
Immunogen SequenceSALTMFISTEQSERDSATAY RMTVEVLGTVIGTAIQGQIV GQAKAPCLQDQNGSVVVSEV ANRTQSTASLKDTQNA
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 170-245 of mouse Mfsd2a (NP_083938.2).

Additional Info

Tissue Specificity Widely expressed. Exhibits an oscillatory pattern of expression in brown adipose tissue and liver consistent with a circadian rhythm. Enriched in brain micro-vessels, where it is specifically present in endothelium constituting the blood-brain barrier (at protein level).
Post Translational Modifications N-glycosylated.
Function Sodium-dependent lysophosphatidylcholine (LPC) symporter, which plays an essential role for blood-brain barrier formation and function. Specifically expressed in endothelium of the blood-brain barrier of micro-vessels and transports LPC into the brain. Transport of LPC is essential because it constitutes the major mechanism by which docosahexaenoic acid (DHA), an omega-3 fatty acid that is essential for normal brain growth and cognitive function, enters the brain. Transports LPC carrying long-chain fatty acids such LPC oleate and LPC palmitate with a minimum acyl chain length of 14 carbons. Does not transport docosahexaenoic acid in unesterified fatty acid. Not required for central nervous system vascular morphogenesis.
Protein Name Sodium-Dependent Lysophosphatidylcholine Symporter 1
Nls1
Sodium-Dependent Lpc Symporter 1
Major Facilitator Superfamily Domain-Containing Protein 2a
Mfsd2a
Database Links Reactome: R-MMU-1483191
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Endoplasmic Reticulum Membrane
Alternative Antibody Names Anti-Sodium-Dependent Lysophosphatidylcholine Symporter 1 antibody
Anti-Nls1 antibody
Anti-Sodium-Dependent Lpc Symporter 1 antibody
Anti-Major Facilitator Superfamily Domain-Containing Protein 2a antibody
Anti-Mfsd2a antibody
Anti-Mfsd2a antibody
Anti-Mfsd2 antibody
Anti-Nls1 antibody

Information sourced from Uniprot.org

Citations

Product Review

!