| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MFN1 |
| Gene ID: | 55669 |
| Uniprot ID: | MFN1_HUMAN |
| Immunogen Region: | 622-741 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 622-741 of human Mitofusin-1/MFN1 (NP_284941.2). |
| Immunogen Sequence: | YGALYLYERLSWTTHAKERA FKQQFVNYATEKLRMIVSST SANCSHQVKQQIATTFARLC QQVDITQKQLEEEIARLPKE IDQLEKIQNNSKLLRNKAVQ LENELENFTKQFLPSSNEES |
| Tissue Specificity | Detected in kidney and heart (at protein level). Ubiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers. |
| Post Translational Modifications | Ubiquitinated by non-degradative ubiquitin by PRKN. Deubiquitination by USP30 inhibits mitochondrial fusion. Ubiquitinated by MARCHF5. When mitochondria are depolarized and dysfunctional, it is ubiquitinated by a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that contains FBXO7 and PRKN. |
| Function | Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion. Membrane clustering requires GTPase activity. It may involve a major rearrangement of the coiled coil domains. Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks (in vitro). Has low GTPase activity. |
| Protein Name | Mitofusin-1 Fzo Homolog Transmembrane Gtpase Mfn1 |
| Database Links | Reactome: R-HSA-5205685 Reactome: R-HSA-9013419 Reactome: R-HSA-983231 |
| Cellular Localisation | Mitochondrion Outer Membrane Multi-Pass Membrane Protein Isoform 2: Cytoplasm |
| Alternative Antibody Names | Anti-Mitofusin-1 antibody Anti-Fzo Homolog antibody Anti-Transmembrane Gtpase Mfn1 antibody Anti-MFN1 antibody |
Information sourced from Uniprot.org