• Western blot analysis of extracts of 22Rv1 cells, using METTL7A antibody (STJ110500) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using METTL7A rabbit polyclonal antibody (STJ110500) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver using METTL7A rabbit polyclonal antibody (STJ110500) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of L929 cells using METTL7A rabbit polyclonal antibody (STJ110500) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HepG2 cells using METTL7A rabbit polyclonal antibody (STJ110500) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-METTL7A antibody (30-244) (STJ110500)

SKU:
STJ110500

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-METTL7A (30-244) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TMT1A
Gene ID: 25840
Uniprot ID: TMT1A_HUMAN
Immunogen Region: 30-244
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-244 of human METTL7A (NP_054752.3).
Immunogen Sequence: WICKKWFPYFLVRFTVIYNE QMASKKRELFSNLQEFAGPS GKLSLLEVGCGTGANFKFYP PGCRVTCIDPNPNFEKFLIK SIAENRHLQFERFVVAAGEN MHQVADGSVDVVVCTLVLCS VKNQERILREVCRVLRPGGA FYFMEHVAAECSTWNYFWQQ VLDPAWHLLFDGCNLTRESW KALERASFSKLKLQHIQAPL SWELVRPHIYGYAVK
Function N(6)-adenine-methyltransferase able to methylate both DNA and long non-coding RNAs. Methylates adenosine residues at the N6 position of some long non-coding RNAs (lncRNAs). DNA methylation by TMT1A promote osteogenic and odontogenic differentiation by upregulating expression of genes that promote differentiation and survival. Recruits cellular proteins to the lipid droplet for the formation of functional organelles. Acts also as an oncogene in multiple myeloma. (Microbial infection) May be involved in the assembly and release stages of hepatitis C virus (HCV) life cycle and thus play a crucial role in HCV propagation.
Protein Name N6-Adenosine-Methyltransferase Tmt1a
Methyltransferase Tmt1a
Methyltransferase-Like Protein 7a
Protein Aam-B
Thiol Methyltransferase 1a
Database Links Reactome: R-HSA-6798695
Cellular Localisation Lipid Droplet
Endoplasmic Reticulum
Membrane
Inserted In The Er Membrane And Migrates From The Inserted Site To Lipid Droplet
Alternative Antibody Names Anti-N6-Adenosine-Methyltransferase Tmt1a antibody
Anti-Methyltransferase Tmt1a antibody
Anti-Methyltransferase-Like Protein 7a antibody
Anti-Protein Aam-B antibody
Anti-Thiol Methyltransferase 1a antibody
Anti-TMT1A antibody
Anti-METTL7A antibody
Anti-PRO0066 antibody
Anti-UNQ1902 antibody
Anti-PRO4348 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance