Anti-METTL4 antibody (1-190) (STJ111645)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal METTL4 (1-190) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | METTL4 |
| Gene ID | 64863 |
| Uniprot ID | METL4_HUMAN |
| Immunogen Region | 1-190 |
| Immunogen Sequence | MSVVHQLSAGWLLDHLSFIN KINYQLHQHHEPCCRKKEFT TSVHFESLQMDSVSSSGVCA AFIASDSSTKPENDDGGNYE MFTRKFVFRPELFDVTKPYI TPAVHKECQQSNEKEDLMNG VKKEISISIIGKKRKRCVVF NQGELDAMEYHTKIRELILD GSLQLIQEGLKSGFLYPLFE KQDKGSKPIT |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human METTL4 (NP_073751.3). |
Additional Info
| Function | N(6)-adenine-specific methyltransferase that can methylate both RNAs and DNA. Acts as a N(6)-adenine-specific RNA methyltransferase by catalyzing formation of N6,2'-O-dimethyladenosine (m6A(m)) on internal positions of U2 small nuclear RNA (snRNA): methylates the 6th position of adenine residues with a pre-deposited 2'-O-methylation. Internal m6A(m) methylation of snRNAs regulates RNA splicing. Also able to act as a N(6)-adenine-specific DNA methyltransferase by mediating methylation of DNA on the 6th position of adenine (N(6)-methyladenosine). The existence of N(6)-methyladenosine (m6A) on DNA is however unclear in mammals, and additional evidences are required to confirm the role of the N(6)-adenine-specific DNA methyltransferase activity of METTL4 in vivo. Acts as a regulator of mitochondrial transcript levels and mitochondrial DNA (mtDNA) copy number by mediating mtDNA N(6)-methylation: m6A on mtDNA reduces transcription by repressing TFAM DNA-binding and bending. N(6)-methyladenosine deposition by METTL4 regulates Polycomb silencing by triggering ubiquitination and degradation of sensor proteins ASXL1 and MPND, leading to inactivation of the PR-DUB complex and subsequent preservation of Polycomb silencing. |
| Protein Name | N(6-Adenine-Specific Methyltransferase Mettl4Methyltransferase-Like Protein 4N(6-Adenine-Specific Dna Methyltransferase Mettl4Snrna2'-O-Methyladenosine-N(6--Methyltransferase Mettl4 |
| Database Links | |
| Cellular Localisation | NucleusCytoplasmCytosolMitochondrion Matrix |
| Alternative Antibody Names | Anti-N(6-Adenine-Specific Methyltransferase Mettl4 antibodyAnti-Methyltransferase-Like Protein 4 antibodyAnti-N(6-Adenine-Specific Dna Methyltransferase Mettl4 antibodyAnti-Snrna antibodyAnti-2'-O-Methyladenosine-N(6--Methyltransferase Mettl4 antibodyAnti-METTL4 antibody |
Information sourced from Uniprot.org