Anti-Methionine Aminopeptidase 2 antibody [R05-2T3] (STJA0036976)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human Methionine Aminopeptidase 2
General Information
| Short Description | Rabbit monoclonal anti-Methionine Aminopeptidase 2 for use in WB, IHC-P, ICC, IF and FC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ICC/IF/FC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R05-2T3 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Chromatography |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200FC 1:50-1:100 |
| Formulation | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | METAP2 |
| Gene ID | 10988 |
| Uniprot ID | MAP2_HUMAN |
| Immunogen | A synthesized peptide derived from human Methionine Aminopeptidase 2 |
Additional Info
| Post Translational Modifications | Contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. O-glycosylation is required for EIF2S1 binding. |
| Function | Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis. |
| Protein Name | Methionine Aminopeptidase 2Map 2Metap 2Initiation Factor 2-Associated 67 Kda GlycoproteinP67P67eif2Peptidase M |
| Database Links | Reactome: R-HSA-2514859 |
| Cellular Localisation | CytoplasmAbout 30% Of Expressed Metap2 Associates With Polysomes |
| Alternative Antibody Names | Anti-Methionine Aminopeptidase 2 antibodyAnti-Map 2 antibodyAnti-Metap 2 antibodyAnti-Initiation Factor 2-Associated 67 Kda Glycoprotein antibodyAnti-P67 antibodyAnti-P67eif2 antibodyAnti-Peptidase M antibodyAnti-METAP2 antibodyAnti-MNPEP antibodyAnti-P67EIF2 antibody |
Information sourced from Uniprot.org