Anti-MerTK/Tyro3 antibody (716-765 aa) (STJ94099)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human MER/SKY at the amino acid range 716-765
General Information
| Short Description | Rabbit polyclonal anti-Tyrosine-protein kinase Mer and Tyrosine-protein kinase receptor TYRO3 (716-765 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:20000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TYRO3MERTK |
| Gene ID | 730110461 |
| Uniprot ID | TYRO3_HUMANMERTK_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human MER/SKY at the amino acid range 716-765 |
| Immunogen Region | 716-765 aa |
| Specificity | MerTK/Tyro3 Polyclonal Antibody detects endogenous levels of MerTK/Tyro3 protein. |
Additional Info
| Post Translational Modifications | Autophosphorylated on Tyr-749, Tyr-753 and Tyr-754 in the activation loop allowing full activity. Autophosphorylated on Tyr-872 leading to recruitment of downstream partners of the signaling cascade such as PLCG2. |
| Function | Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. |
| Protein Name | Tyrosine-Protein Kinase MerProto-Oncogene C-MerReceptor Tyrosine Kinase Mertk |
| Database Links | Reactome: R-HSA-202733 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Tyrosine-Protein Kinase Mer antibodyAnti-Proto-Oncogene C-Mer antibodyAnti-Receptor Tyrosine Kinase Mertk antibodyAnti-MERTK antibodyAnti-MER antibody |
Information sourced from Uniprot.org