| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MERTK |
| Gene ID: | 10461 |
| Uniprot ID: | MERTK_HUMAN |
| Immunogen Region: | 26-999 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 26-499 of human MERTK (NP_006334.2). |
| Immunogen Sequence: | REEAKPYPLFPGPFPGSLQT DHTPLLSLPHASGYQPALMF SPTQPGRPHTGNVAIPQVTS VESKPLPPLAFKHTVGHIIL SEHKGVKFNCSISVPNIYQD TTISWWKDGKELLGAHHAIT QFYPDDEVTAIIASFSITSV QRSDNGSYICKMKINNEEIV SDPIYIEVQGLPHFTKQPES MNVTRNTAFNLTCQAVGPPE PVNIFWVQNSSRVNEQPEKS PSVLTVPGLTEMAVFSCEA |
| Tissue Specificity | Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. Highly expressed in testis, ovary, prostate, lung, and kidney, with lower expression in spleen, small intestine, colon, and liver. |
| Post Translational Modifications | Autophosphorylated on Tyr-749, Tyr-753 and Tyr-754 in the activation loop allowing full activity. Autophosphorylated on Tyr-872 leading to recruitment of downstream partners of the signaling cascade such as PLCG2. |
| Function | Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. |
| Protein Name | Tyrosine-Protein Kinase Mer Proto-Oncogene C-Mer Receptor Tyrosine Kinase Mertk |
| Database Links | Reactome: R-HSA-202733 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Tyrosine-Protein Kinase Mer antibody Anti-Proto-Oncogene C-Mer antibody Anti-Receptor Tyrosine Kinase Mertk antibody Anti-MERTK antibody Anti-MER antibody |
Information sourced from Uniprot.org