• Western blot analysis of 293T, using MERS-CoVNucleoprotein antibody (STJ11104812) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MERS-CoV Nucleoprotein antibody (1-413) [S4812RM] (STJ11104812)

SKU:
STJ11104812

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-MERS-CoV Nucleoprotein (1-413) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4812RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1-413
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-413 of coronavirus MERS-CoV Nucleoprotein (YP_009047211.1).
Immunogen Sequence: MASPAAPRAVSFADNNDITN TNLSRGRGRNPKPRAAPNNT VSWYTGLTQHGKVPLTFPPG QGVPLNANSTPAQNAGYWRR QDRKINTGNGIKQLAPRWYF YYTGTGPEAALPFRAVKDGI VWVHEDGATDAPSTFGTRNP NNDSAIVTQFAPGTKLPKNF HIEGTGGNSQSSSRASSLSR NSSRSSSQGSRSGNSTRGTS PGPSGIGAVGGDLLYLDLLN RLQALESGKVKQSQPKVIT
Background Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.

Information sourced from Uniprot.org

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