Western blot analysis of various lysates using Melan-A/MART-1 Rabbit mAb (STJ11107273) at 1:20000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Negative control (NC) : OVCAR-3<br>Exposure time: 3s.

Anti-Melan-A/MART-1 antibody (11-110 aa) [SRM5363] (STJ11107273)

SKU:
STJ11107273
€151.80 - €665.40
Processing The item has been added
Enquire For Bulk Order

Covered by St John's Laboratory Guarantee

Applications:WB/ELISA
Host:Rabbit
Reactivity:Human
Note:STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality :Monoclonal
Clone ID :SRM5363
Isotype:IgG
Conjugation:Unconjugated
Concentration:Lot specific
Purification:Affinity purification
Dilution Range:WB:1:20000-1:120000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Formulation:PBS with 0.09% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage Instruction:Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol:MLANA
Gene ID:2315
Uniprot ID:MAR1_HUMAN
Immunogen Region:11-110 aa
Immunogen Sequence:VLLLIGCWYCRRRNGYRALM DKSLHVGTQCALTRRCPQEG FDHRDSKVSLQEKNCEPVVP NAPPAYEKLSAEQSPPPYSP
Specificity:A synthetic peptide corresponding to a sequence within amino acids 11-110 of human Melan-A/MART-1 (NP_005502.1).
Tissue Specificity Expression is restricted to melanoma and melanocyte cell lines and retina.
Post Translational Modifications Acylated.
Function Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.
Protein Name Melanoma Antigen Recognized By T-Cells 1
Mart-1
Antigen Lb39-Aa
Antigen Sk29-Aa
Protein Melan-A
Database Links Reactome: R-HSA-9824585
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Iii Membrane Protein
Golgi Apparatus
Trans-Golgi Network Membrane
Melanosome
Also Found In Small Vesicles And Tubules Dispersed Over The Entire Cytoplasm
A Small Fraction Of The Protein Is Inserted Into The Membrane In An Inverted Orientation
Inversion Of Membrane Topology Results In The Relocalization Of The Protein From A Predominant Golgi/Post-Golgi Area To The Endoplasmic Reticulum
Melanoma Cells Expressing The Protein With An Inverted Membrane Topology Are More Effectively Recognized By Specific Cytolytic T-Lymphocytes Than Those Expressing The Protein In Its Native Membrane Orientation
Alternative Antibody Names Anti-Melanoma Antigen Recognized By T-Cells 1 antibody
Anti-Mart-1 antibody
Anti-Antigen Lb39-Aa antibody
Anti-Antigen Sk29-Aa antibody
Anti-Protein Melan-A antibody
Anti-MLANA antibody
Anti-MART1 antibody

Information sourced from Uniprot.org

Related Products

Model Name Host Clonality Reactivity Application
STJ11109241 Anti-Melan-A/MART-1 antibody [SRM14511] (STJ11109241) Rabbit Monoclonal Human WB/IF/ICC/IHC-P/ELISA
STJ16101844 Anti-MART-1 (MELAN A) antibody [M2-9E3] (STJ16101844) Mouse Monoclonal Human/Mouse/Rat IHC-F/IHC-P
STJP018253 Human Melan-A/MART-1 protein (Recombinant) (His-Tag) (STJP018253) E.coli SDS-PAGE
STJA0042209 Anti-MART-1 antibody [IHC418] (STJA0042209) Mouse Monoclonal Human IHC-p
STJ16100387 Anti-Mart-1 antibody [A103] (STJ16100387) Mouse Monoclonal Human IHC-P

Related Products

!