• Western blot detection of MEK1/2 in A549, MCF7 and Hela cell lysates using MEK1/2 mouse mAb (dilution 1:500).Predicted band size:45kDa.Observed band size:45kDa.

Anti-MEK1 antibody [6F10-D7-G6] (STJ99087)

SKU:
STJ99087

Current Stock:
Host: Mouse
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-MAP2K1 is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: 6F10-D7-G6
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAP2K2
Gene ID: 5605
Uniprot ID: MP2K2_HUMAN
Specificity: This antibody detects endogenous levels of MEK1/2 and does not cross-react with related proteins.
Immunogen: Recombinant human MEK1 protein.
Function Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases. Activates BRAF in a KSR1 or KSR2-dependent manner.by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation.
Protein Name Dual Specificity Mitogen-Activated Protein Kinase Kinase 2
Map Kinase Kinase 2
Mapkk 2
Erk Activator Kinase 2
Mapk/Erk Kinase 2
Mek 2
Database Links Reactome: R-HSA-112411
Reactome: R-HSA-170968
Reactome: R-HSA-445144
Reactome: R-HSA-5210891
Reactome: R-HSA-5673000
Reactome: R-HSA-5674135
Reactome: R-HSA-5674499
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-9649948
Reactome: R-HSA-9652169
Reactome: R-HSA-9656223
Cellular Localisation Cytoplasm
Membrane
Peripheral Membrane Protein
Membrane Localization Is Probably Regulated By Its Interaction With Ksr1
Alternative Antibody Names Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 2 antibody
Anti-Map Kinase Kinase 2 antibody
Anti-Mapkk 2 antibody
Anti-Erk Activator Kinase 2 antibody
Anti-Mapk/Erk Kinase 2 antibody
Anti-Mek 2 antibody
Anti-MAP2K2 antibody
Anti-MEK2 antibody
Anti-MKK2 antibody
Anti-PRKMK2 antibody

Information sourced from Uniprot.org