| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Mediator of RNA polymerase II transcription subunit 15 (510-590 aa) is suitable for use in Western Blot and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 ELISA 1:5000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MED15 |
| Gene ID: | 51586 |
| Uniprot ID: | MED15_HUMAN |
| Immunogen Region: | 510-590 aa |
| Specificity: | MED15 Polyclonal Antibody detects endogenous levels of protein. |
| Immunogen: | Synthesized peptide derived from the human protein at the amino acid range 510-590 |
| Post Translational Modifications | Ubiquitinated by TRIM11, leading to proteasomal degradation. |
| Function | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway. |
| Protein Name | Mediator Of Rna Polymerase Ii Transcription Subunit 15 Activator-Recruited Cofactor 105 Kda Component Arc105 Ctg Repeat Protein 7a Mediator Complex Subunit 15 Positive Cofactor 2 Glutamine/Q-Rich-Associated Protein Pc2 Glutamine/Q-Rich-Associated Protein Tpa-Inducible Gene 1 Protein Tig-1 Trinucleotide Repeat-Containing Gene 7 Protein |
| Database Links | Reactome: R-HSA-1989781 Reactome: R-HSA-212436 Reactome: R-HSA-381340 Reactome: R-HSA-9833110 |
| Cellular Localisation | Cytoplasm Nucleus |
| Alternative Antibody Names | Anti-Mediator Of Rna Polymerase Ii Transcription Subunit 15 antibody Anti-Activator-Recruited Cofactor 105 Kda Component antibody Anti-Arc105 antibody Anti-Ctg Repeat Protein 7a antibody Anti-Mediator Complex Subunit 15 antibody Anti-Positive Cofactor 2 Glutamine/Q-Rich-Associated Protein antibody Anti-Pc2 Glutamine/Q-Rich-Associated Protein antibody Anti-Tpa-Inducible Gene 1 Protein antibody Anti-Tig-1 antibody Anti-Trinucleotide Repeat-Containing Gene 7 Protein antibody Anti-MED15 antibody Anti-ARC105 antibody Anti-CTG7A antibody Anti-PCQAP antibody Anti-TIG1 antibody Anti-TNRC7 antibody |
Information sourced from Uniprot.org