• Western blot of mouse whole brain lysate showing specific immunolabeling of the MeCP2 protein at ~75 kDa.
  • Immunofluorescence of a section of rat cerebellum showing specific labeling of MeCP2 (STJA0003679, 1:1000, red) in nuclei of neurons and specific labeling of GFAP ( cat. STJA0003659, 1:5000, green) in the network of astroglial cells and projections of Bergmann glia, and Hoechst staining of nuclear DNA.
  • Immunostaining of a section of rat hippocampus showing specific nuclear immunolabeling of MeCP2 (1205-MECP2, red, 1:1000) and FOX3 (green). The blue is DAPI staining of nuclear DNA.
  • Immunofluorescence of a section of rat cerebellum labeled with anti-MeCP2 (1205-MECP2, 1:500, red) , colabeled with anti-GFAP ( cat. 620-GFAP, 1:5000, green) , and DAPI staining of nuclear DNA. The anti-GFAP labels the astrocytic cells and the processes of Bergmann glia in the molecular layer.

Anti-MECP2 antibody (Full Length) [5H12] (STJA0003679)

SKU:
STJA0003679-100

£442.50
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Host: Mouse
Applications: WB/IHC/ICC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-MeCP2 (Full Length) is suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry research applications.
Clonality: Monoclonal
Clone ID: 5H12
Conjugation: Unconjugated
Isotype: IgG2b
Formulation: 100 ul in PBS + 50% Glycerol and 5 mM Sodium Azide
Purification: This antibody was protein g purified culture from supernatant.
Dilution Range: WB 1:2000
IHC 1:500-1:5000
ICC 1:1000-1:5000
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MECP2
Gene ID: 4204
Uniprot ID: MECP2_HUMAN
Immunogen Region: Full Length
Immunogen: Full length human recombinant MeCP2.
Tissue Specificity Present in all adult somatic tissues tested.
Post Translational Modifications Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation.
Function Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).
Protein Name Methyl-Cpg-Binding Protein 2
Mecp-2 Protein
Mecp2
Database Links Reactome: R-HSA-8986944
Reactome: R-HSA-9022534
Reactome: R-HSA-9022535
Reactome: R-HSA-9022537
Reactome: R-HSA-9022538
Reactome: R-HSA-9022692
Reactome: R-HSA-9022699
Reactome: R-HSA-9022702
Reactome: R-HSA-9022707
Reactome: R-HSA-9022927
Reactome: R-HSA-9725371
Cellular Localisation Nucleus
Colocalized With Methyl-Cpg In The Genome
Colocalized With Tbl1x To The Heterochromatin Foci
Alternative Antibody Names Anti-Methyl-Cpg-Binding Protein 2 antibody
Anti-Mecp-2 Protein antibody
Anti-Mecp2 antibody
Anti-MECP2 antibody

Information sourced from Uniprot.org

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