Anti-MDM4 antibody (336-385 aa) (STJ94058)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human MDM4 at the amino acid range 336-385
General Information
| Short Description | Rabbit polyclonal anti-Protein Mdm4 (336-385 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MDM4 |
| Gene ID | 4194 |
| Uniprot ID | MDM4_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human MDM4 at the amino acid range 336-385 |
| Immunogen Region | 336-385 aa |
| Specificity | MDMX Polyclonal Antibody detects endogenous levels of MDMX protein. |
Additional Info
| Post Translational Modifications | Phosphorylated. Phosphorylation at Ser-367 promotes interaction with YWHAG and subsequent ubiquitination and degradation. Phosphorylation at Ser-342 also induces ubiquitination and degradation but to a lower extent. Ubiquitinated and degraded by MDM2. Deubiquitination by USP2 on the other hand stabilizes the MDM4 protein. |
| Function | Along with MDM2, contributes to TP53 regulation. Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions. |
| Protein Name | Protein Mdm4Double Minute 4 ProteinMdm2-Like P53-Binding ProteinProtein MdmxP53-Binding Protein Mdm4 |
| Database Links | Reactome: R-HSA-2559580Reactome: R-HSA-2559585Reactome: R-HSA-5689880Reactome: R-HSA-6804756Reactome: R-HSA-6804757Reactome: R-HSA-6804760Reactome: R-HSA-69541 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Protein Mdm4 antibodyAnti-Double Minute 4 Protein antibodyAnti-Mdm2-Like P53-Binding Protein antibodyAnti-Protein Mdmx antibodyAnti-P53-Binding Protein Mdm4 antibodyAnti-MDM4 antibodyAnti-MDMX antibody |
Information sourced from Uniprot.org