• Western blot analysis of extracts of various cell lines, using MDH2 antibody (STJ115477) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MDH2 antibody (59-338) (STJ115477)

SKU:
STJ115477

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MDH2
Gene ID: 4191
Uniprot ID: MDHM_HUMAN
Immunogen Region: 59-338
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 59-338 of human MDH2 (NP_005909.2).
Immunogen Sequence: AHTPGVAADLSHIETKAAVK GYLGPEQLPDCLKGCDVVVI PAGVPRKPGMTRDDLFNTNA TIVATLTAACAQHCPEAMIC VIANPVNSTIPITAEVFKKH GVYNPNKIFGVTTLDIVRAN TFVAELKGLDPARVNVPVIG GHAGKTIIPLISQCTPKVDF PQDQLTALTGRIQEAGTEVV KAKAGAGSATLSMAYAGARF VFSLVDAMNGKEGVVECSFV KSQETECTYFSTPLLLGKK
Post Translational Modifications Acetylation is enhanced by up to 67% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with the appearance of tri- and tetraacetylations. Glucose also increases acetylation by about 60%.
Protein Name Malate Dehydrogenase - Mitochondrial
Database Links Reactome: R-HSA-71403
Reactome: R-HSA-9837999
Reactome: R-HSA-9856872
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Malate Dehydrogenase - Mitochondrial antibody
Anti-MDH2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance