| Host: |
Goat |
| Applications: |
Pep-ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-MDA5/Ifih1-Mouse (Internal) is suitable for use in ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
ELISA-antibody detection limit dilution 1:16000. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_082111.1 |
| Immunogen Sequence: |
KQKLRNNDLKKQN |
| Post Translational Modifications | During apoptosis, processed into 3 cleavage products. The helicase-containing fragment, once liberated from the CARD domains, translocate from the cytoplasm to the nucleus. The processed protein significantly sensitizes cells to DNA degradation. Sumoylated. Sumoylation positively regulates its role in type I interferon induction and is enhanced by PIAS2-beta. Ubiquitinated by RNF125, leading to its degradation by the proteasome. USP17/UPS17L2-dependent deubiquitination positively regulates the receptor. Ubiquitinated by TRIM25 via 'Lys-63'-linked ubiquitination, promoting activation of IFIH1/MDA5. Ubiquitinated by TRIM40 via 'Lys-48'-linked ubiquitination.leading to proteasomal degradation. |
| Function | Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include mRNA lacking 2'-O-methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs).IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV), mengo encephalomyocarditis virus (ENMG), and theiler's murine encephalomyelitis virus (TMEV). Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines. |
| Protein Name | Interferon-Induced Helicase C Domain-Containing Protein 1Helicase With 2 Card DomainsHelicardInterferon Induced With Helicase C Domain Protein 1Melanoma Differentiation-Associated Protein 5Mda-5Rig-I-Like Receptor 2Rlr-2 |
| Database Links | Reactome: R-MMU-5689880 |
| Cellular Localisation | CytoplasmNucleusMay Be Found In The NucleusDuring Apoptosis |
| Alternative Antibody Names | Anti-Interferon-Induced Helicase C Domain-Containing Protein 1 antibodyAnti-Helicase With 2 Card Domains antibodyAnti-Helicard antibodyAnti-Interferon Induced With Helicase C Domain Protein 1 antibodyAnti-Melanoma Differentiation-Associated Protein 5 antibodyAnti-Mda-5 antibodyAnti-Rig-I-Like Receptor 2 antibodyAnti-Rlr-2 antibodyAnti-Ifih1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
