Anti-MCTS1 antibody (N-Term) (STJ72898)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-MCTS1 (N-Term) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human/Dog/Pig/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 19kDa band observed in lysates of cell lines Jurkat and MOLT4 (calculated MW of 20.6kDa according to NP_054779.1 and NP_001131026.1). Recommended concentration: 0.3-1µg/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | MCTS1 |
| Gene ID | 28985 |
| Uniprot ID | MCTS1_HUMAN |
| Accession Number | NP_054779.1; NP_001131026.1 |
| Immunogen Region | N-Term |
| Immunogen Sequence | DEKENVSNCIQLKTS |
| Specificity | This antibody is expected to recognize both reported isosoforms (NP_054779.1; NP_001131026.1). |
Additional Info
| Post Translational Modifications | Phosphorylation is critical for stabilization and promotion of cell proliferation. |
| Function | Translation regulator forming a complex with DENR to promote translation reinitiation. Translation reinitiation is the process where the small ribosomal subunit remains attached to the mRNA following termination of translation of a regulatory upstream ORF (uORF), and resume scanning on the same mRNA molecule to initiate translation of a downstream ORF, usually the main ORF (mORF). The MCTS1/DENR complex is pivotal to two linked mechanisms essential for translation reinitiation. Firstly, the dissociation of deacylated tRNAs from post-termination 40S ribosomal complexes during ribosome recycling. Secondly, the recruitment in an EIF2-independent manner of aminoacylated initiator tRNA to P site of 40S ribosomes for a new round of translation. This regulatory mechanism governs the translation of more than 150 genes which translation reinitiation is MCTS1/DENR complex-dependent. Consequently, modulates various unrelated biological processes including cell cycle regulation and DNA damage signaling and repair. Notably, it positively regulates interferon gamma immunity to mycobacteria by enhancing the translation of JAK2. |
| Protein Name | Malignant T-Cell-Amplified Sequence 1Mct-1Multiple Copies T-Cell Malignancies |
| Cellular Localisation | CytoplasmNuclear Relocalization After Dna Damage |
| Alternative Antibody Names | Anti-Malignant T-Cell-Amplified Sequence 1 antibodyAnti-Mct-1 antibodyAnti-Multiple Copies T-Cell Malignancies antibodyAnti-MCTS1 antibodyAnti-MCT1 antibody |
Information sourced from Uniprot.org