Anti-MCM6 antibody (562-821) (STJ24526)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal MCM6 (562-821) antibody for use in WB, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MCM6 |
| Gene ID | 4175 |
| Uniprot ID | MCM6_HUMAN |
| Immunogen Region | 562-821 |
| Immunogen Sequence | SIDRVYSLDDIRRYLLFARQ FKPKISKESEDFIVEQYKHL RQRDGSGVTKSSWRITVRQL ESMIRLSEAMARMHCCDEVQ PKHVKEAFRLLNKSIIRVET PDVNLDQEEEIQMEVDEGAG GINGHADSPAPVNGINGYNE DINQESAPKASLRLGFSEYC RISNLIVLHLRKVEEEEDES ALKRSELVNWYLKEIESEID SEEELINKKRIIEKVIHRLT HYDHVLIELTQAGLKGSTE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 562-821 of human MCM6 (NP_005906.2). |
Additional Info
| Post Translational Modifications | O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. |
| Function | Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. |
| Protein Name | Dna Replication Licensing Factor Mcm6P105mcm |
| Database Links | Reactome: R-HSA-176187Reactome: R-HSA-176974Reactome: R-HSA-68867Reactome: R-HSA-68949Reactome: R-HSA-68962Reactome: R-HSA-69052 |
| Cellular Localisation | NucleusChromosomeBinds To Chromatin During G1 And Detaches From It During S Phase |
| Alternative Antibody Names | Anti-Dna Replication Licensing Factor Mcm6 antibodyAnti-P105mcm antibodyAnti-MCM6 antibody |
Information sourced from Uniprot.org