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Anti-MCM2 antibody [2B3] (STJ97871)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenPurified recombinant fragment of human BM28 expressed in E. Coli.
General Information
| Short Description | Mouse monoclonal anti-DNA replication licensing factor MCM2 for use in WB, IHC, IF, FC and ELISA in Human, Mouse, Rat and Monkey samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/FC/ELISA |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat/Monkey |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 2B3 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | WB 1:500-1:2000IHC 1:200-1:1000FC 1:200-1:400ELISA 1:10000IF 1:50-200 |
| Formulation | Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MCM2 |
| Gene ID | 4171 |
| Uniprot ID | MCM2_HUMAN |
| Immunogen | Purified recombinant fragment of human BM28 expressed in E. Coli. |
| Specificity | BM28 Monoclonal Antibody detects endogenous levels of BM28 protein. |
Additional Info
| Function | Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis. |
| Protein Name | Dna Replication Licensing Factor Mcm2Minichromosome Maintenance Protein 2 HomologNuclear Protein Bm28 |
| Database Links | Reactome: R-HSA-176187Reactome: R-HSA-176974Reactome: R-HSA-68867Reactome: R-HSA-68949Reactome: R-HSA-68962Reactome: R-HSA-69052Reactome: R-HSA-9825895 |
| Cellular Localisation | NucleusChromosomeAssociated With Chromatin Before The Formation Of Nuclei And Detaches From It As Dna Replication Progresses |
| Alternative Antibody Names | Anti-Dna Replication Licensing Factor Mcm2 antibodyAnti-Minichromosome Maintenance Protein 2 Homolog antibodyAnti-Nuclear Protein Bm28 antibodyAnti-MCM2 antibodyAnti-BM28 antibodyAnti-CCNL1 antibodyAnti-CDCL1 antibodyAnti-KIAA0030 antibody |
Information sourced from Uniprot.org